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Přirozené antioxidanty v potravinách jako prevence oxidativního stresu v organismu
Kaserová, Natálie
The bachelor thesis is focused on natural antioxidants and their fight against oxidative stress in the organism. The first part is about the oxidative stress, what causes it, and its negative or positive effects on the human body. The connection between free radicals and the development of oxidative stress is outlined. The second part of the literature review is devoted to the antioxidants themselves, both natural and natively occurring in the human organism. The low-molecular organic molecules of plants are described in more detail, which exhibit significant antioxidant activity and constitute a substantial part of our diet. The foods or beverages with high content of antioxidant substances are mentioned in the last part of bachelor thesis. This chapter deals with the foods such as vegetables, fruits, nuts and even cereals. Furthermore, from the beverages are presented the tea, coffee, beer, and wine.
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Molecular bases of sensitivity to electron transport chain inhibition-induced cell death
Blecha, Jan
1 Abstract in English Mitochondrial electron transport chain (ETC) targeting shows a great promise in cancer therapy. However, why modern ETC-targeted compounds are tolerated on the organismal level and what are the molecular reasons for this tolerance remains unclear. Most somatic cells are in a non-proliferative state, and features associated with the ETC in quiescence might therefore contribute to specificity. Thus, we investigated the ETC status and the role of two major consequences of ETC blockade, reactive oxygen species (ROS) generation and inhibition of ATP production, in cell death induction in breast cancer cells and in proliferating and quiescent non-transformed cells. First, we characterised the effect of a newly developed ETC inhibitor mitochondria- targeted tamoxifen (MitoTam) in in vitro and in vivo tumour models of breast cancer with varying status of the Her2 oncogene. We document that Her2high cells and tumours have increased assembly of respiratory supercomplexes (SCs) and increased complex I-driven respiration in vitro and in vivo. They are also highly sensitive to MitoTam. Unlike the parental compound tamoxifen, MitoTam efficiently suppressed experimental Her2high tumours without systemic toxicity. Mechanistically, MitoTam inhibits complex I- driven respiration and disrupts respiratory...
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Molecular bases of sensitivity to electron transport chain inhibition-induced cell death
Blecha, Jan
1 Abstract in English Mitochondrial electron transport chain (ETC) targeting shows a great promise in cancer therapy. However, why modern ETC-targeted compounds are tolerated on the organismal level and what are the molecular reasons for this tolerance remains unclear. Most somatic cells are in a non-proliferative state, and features associated with the ETC in quiescence might therefore contribute to specificity. Thus, we investigated the ETC status and the role of two major consequences of ETC blockade, reactive oxygen species (ROS) generation and inhibition of ATP production, in cell death induction in breast cancer cells and in proliferating and quiescent non-transformed cells. First, we characterised the effect of a newly developed ETC inhibitor mitochondria- targeted tamoxifen (MitoTam) in in vitro and in vivo tumour models of breast cancer with varying status of the Her2 oncogene. We document that Her2high cells and tumours have increased assembly of respiratory supercomplexes (SCs) and increased complex I-driven respiration in vitro and in vivo. They are also highly sensitive to MitoTam. Unlike the parental compound tamoxifen, MitoTam efficiently suppressed experimental Her2high tumours without systemic toxicity. Mechanistically, MitoTam inhibits complex I- driven respiration and disrupts respiratory...
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Molecular bases of sensitivity to electron transport chain inhibition-induced cell death
Blecha, Jan ; Rohlena, Jakub (advisor) ; Brábek, Jan (referee) ; Pecinová, Alena (referee)
1 Abstract in English Mitochondrial electron transport chain (ETC) targeting shows a great promise in cancer therapy. However, why modern ETC-targeted compounds are tolerated on the organismal level and what are the molecular reasons for this tolerance remains unclear. Most somatic cells are in a non-proliferative state, and features associated with the ETC in quiescence might therefore contribute to specificity. Thus, we investigated the ETC status and the role of two major consequences of ETC blockade, reactive oxygen species (ROS) generation and inhibition of ATP production, in cell death induction in breast cancer cells and in proliferating and quiescent non-transformed cells. First, we characterised the effect of a newly developed ETC inhibitor mitochondria- targeted tamoxifen (MitoTam) in in vitro and in vivo tumour models of breast cancer with varying status of the Her2 oncogene. We document that Her2high cells and tumours have increased assembly of respiratory supercomplexes (SCs) and increased complex I-driven respiration in vitro and in vivo. They are also highly sensitive to MitoTam. Unlike the parental compound tamoxifen, MitoTam efficiently suppressed experimental Her2high tumours without systemic toxicity. Mechanistically, MitoTam inhibits complex I- driven respiration and disrupts respiratory...
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Defence of organism against reactive oxide substances and the role of nutrition in it
BENDLOVÁ, Radka
Reactive oxygen and nitrogen species along with environmental toxins are the most frequently cited sources of pathological effects on physiological proceses in man and on the development of degenerative diseases (diseases of civilization). Numerous free radicals commonly occur in the organism because they are part of physiological processes (physical exercive, food processing, the body's immune defence). On one hand highly reactive oxygen species play an important role in antimicrobial protection of the human body, helping to kill microorganismus during phagocytosis. Other side of oxidative stress (overproduction of reactive oxygenates) participates damage to proteins, lipids and nucleic acids. Oxidative damage to biomolecules has to be counteracted by antioxidant defense. Sufficient level of antioxidants in biological tissues act protectively. If we follow the recommended quantity and composition of food rich in antioxidants we can substantially contribute to improving our health and reduce the risk of manifestation of the serious diseases, so called civilization diseases.
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